Comparison of Activity and Inhibitory Mechanism between (+)-Catechin and Water Extract of Gambier (Uncaria Gambir Roxb.) Against Some Bacteria

Muhammad Yanis Musdja, Mustikaning Ayu Hapsari, Andria Agusta


Gambier (Uncaria gambier Roxb) has been used by many people in Asia as anti-microbial, anti-diarrhea, and agent for the treatment of burns. Gambier is also one component for chewing medicine to strengthen teeth and antimicrobial. This research aims to compare the antibacterial activity as well as the mechanism of inhibition between (+)-catechins and gambier extract, as the basis for choosing a substance to be developed as antimicrobial drug. (+)-Catechin was extracted from gambier, the result of (+)-catechin which obtained through isolation was compared with (+)-catechin standard by using thin layer chromatography. The anti-bacterial activity tests of (+)-catechin and extract of gambier were carried out by using the method of microdilution against some bacteria, namely, Staphylococcus epidermidis, S.aureus, S. mutans, S. viridans and Bacillus subtilis. For the detection of the mechanism of bacterial cellular destruction, the leakage of proteins and nucleic acids was observed by using UV-Visible spectrophotometer. In addition, the leakage of ions Ca2+ and K+ was observed by using Atomic Absorption Spectrophometer (AAS). Meanwhile, the cellular morphological changes was observed by using scanning electron microscopy (SEM).Isolation results of (+)-catechin from gambier was obtained the yield 22.55%. The results of antibacterial activity of catechins was obtained minimum inhibitory concentration (MIC) values against S. epidermidis, S. mutans, and S. viridans was 5.5 mg/ mL, 8 mg/ mL, and 8 mg/ mL, respectively. While against S. aureus and B. subtilis the MIC values were not obtained up to 25 mg/ mL. The MIC of aqueous extract of gambier against the S. epidermidis was 22.5 mg/ mL. The MIC was not obtained against the other four bacteria until the concentration of 25 mg/ mL.The leakage of protein, nucleic acid as well as K+ and Ca2+ ions were observed at 1 MIC and 2 MIC. T


Catechin; Gambier; Antimicrobial; and Gram Positive Bacteria

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Nurliayana I, Yusoff NZM, Ahmad R, 2016, Quantification of Catechin in Leaves and Stems of Malaysian Uncaria Gambir (Hunter) Roxb. By Hplc-Dad, Malaysian Journal of Analytical Sciences, 20(3),p. 567 – 572, DOI: mjas-2016-2003-15

Anggraini T, Tai A, Yoshino T and Itani T, 2011, Antioxidative activity and catechin content of four kinds of Uncaria gambir extracts from West Sumatra, Indonesia, African Journal of Biochemistry Research, 5(1), p. 33-38.

Heitzman EM, Neto CC, Winiaz E, Vaisberg AJ and Hammond GB, 2005, Ethnobotany, phytochemistry and pharmacology of Uncaria (Rubiaceae), Phytochemistry, 66, p.5-39

Taniguchi S, Kuroda K, Yoshikado N, Doi KI, Tanabe M, Shibata T, Yoshida T and Hatano T, 2008, New Dimeric Flavans From Gambir, An Extract of Uncaria gambir, The Japan Institute of Heterocyclic Chemistry, Okayama University

Pambayun R, Murdijati G, Slamet S and Kapti R, 2007, Kandungan Fenol dan Sifat Antibakteri dari Berbagai Jenis Ekstrak Produk Gambir (Uncaria gambir Roxb), Majalah Farmasi Indonesia, 18(3), p.141-146 (in bahasa).

Voravuthikunchai S, Amornrat L, Wanpen J, Trechada S, Souwalak P and Thanomjit S, 2004, Effective Medicinal Plants Againts Enterohaemorrhagic Escherichia coli O157:H7, Journal of Ethnopharmacology, 94, p. 49-54.

Fatimah, Musdja MY, Sulistiawati F, 2010, Formulasi Tablet Hisap Kombinasi ekstrak etanol 70% Daun Sirih (Piper betle, L) dan Gambir (Uncaria gambir, Roxb) dengan variasi konsentrasi Maltodekstrin sebagai Pengikat Menggunakan Metode Cetak langsung dan Uji Efek Imunomodulator, BSc Thesis, UIN Jakarta, (in bahasa).

Suppakul P, Ead NS and Phoopuritham P, Antimicrobial and Antioxidant Activities of Betel Oil, Kasetsart Journal (Natural Science), 40, p.91 – 100.

Caburian AB and Osi MO, 2010, Characterization and Evaluation of Antimicrobial Activity of the Essential Oil from the Leaves of Piper betle, E-International Scientific Research Journal, 2(1), p.2 – 12

Carson CF, Mee BJ and Riley TV, Mechanism of Action of Tea Tree Oil on Staphylococcus aureus Determined by Time Kill, lyses, Leakage, and Salt Tolerance Assays and Electron Microscopy, Antimicrobial Agent and Chemotherapy, 6, p.1914 – 1920

Amos, 2010, Kandungan Katekin Gambir Sentra Produksi di Indonesia, Jurnal Standardisasi, 12(3), p.149 – 155 (in bahasa)

Das NP and Griffiths LA, 1967, Studies on flavonoid metabolism. Biosynthesis of (+)-[14C]catechin by the plant Uncaria gambir Roxb, Biochemical Journal, 105(1), p.73–77.


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